There are a selection of biofilm growth methods for in-lab testing of antimicrobials against biofilms, where the CDC and MBEC methods are the two most commonly used methods. The current study has looked into determining if merging of the two prominent techniques can provide a more desirable protocol for biofilm growth and testing. Additionally, two antimicrobial agents have been used to assess the strength of the grown biofilms; a standard sodium hypochlorite (SHC) solution as a known biofilm killing agent, and diluted Accelerated Hydrogen Peroxide® (AHP®) concentrate formula as a novel agent against biofilms.
